2BNU
Structural and kinetic basis for heightened immunogenicity of T cell vaccines
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-20 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.991, 60.295, 82.641 |
Unit cell angles | 90.00, 89.54, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.400 |
R-factor | 0.245 |
Rwork | 0.245 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oga |
RMSD bond length | 0.005 |
RMSD bond angle | 1.310 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.460 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.080 | 0.320 |
Number of reflections | 85029 | |
<I/σ(I)> | 24.7 | 4.3 |
Completeness [%] | 93.0 | 62.7 |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | PH 6.8 |