2BNF

The structure of E. coli UMP kinase in complex with UTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Spacegroup name	H 3
Unit cell lengths	141.910, 141.910, 60.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.000 - 2.450
R-factor	0.1836
Rwork	0.184
R-free	0.23110
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.230
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.450	2.450
Rmerge	0.090	0.520
Number of reflections	33118
<I/σ(I)>	10.9	2.2
Completeness [%]	99.4	99
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		pH 8.50