2BMZ
Banana Lectin bound to Xyl-b1,3 Man-a-O-Methyl (XM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.623, 81.623, 146.815 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.990 - 2.400 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bmy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.100 | 2.200 |
| Rmerge | 0.070 | 0.340 |
| Number of reflections | 29782 | |
| <I/σ(I)> | 20 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.25 | 1.275M AMMONIUM SULFATE 30MM CADMIUM CHLORIDE 0.1M TRIS-HCL, PH=8.25 |
