2BMD
high resolution structure of GDP-bound human Rab4a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.140, 63.750, 38.280 |
| Unit cell angles | 90.00, 100.03, 90.00 |
Refinement procedure
| Resolution | 18.290 - 1.800 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.485 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 15669 | |
| <I/σ(I)> | 14.44 | 7.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% (W/V) PEG 8000, 200MM AMMONIUM SULPHATE, 100MM SODIUM CACODYLATE PH6.5 HANGING DROP 20 DEGREE CELSIUS |
