2BMB
X-ray structure of the bifunctional 6-hydroxymethyl-7,8- dihydroxypterin pyrophosphokinase dihydropteroate synthase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-18 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.566, 92.566, 192.401 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.300 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eye PDB ENTRY 1CBK |
RMSD bond length | 0.009 |
RMSD bond angle | 1.212 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.170 | 0.850 |
Number of reflections | 37688 | |
<I/σ(I)> | 22.4 | 3.8 |
Completeness [%] | 99.1 | 91.1 |
Redundancy | 10.7 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 1.17 M NAH2PO4, 0.63 M K2HPO4, PH 6.3 |