2BKX
Structure and kinetics of a monomeric glucosamine-6-phosphate deaminase: missing link of the NagB superfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 120 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.966, 47.898, 71.397 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.400 |
| R-factor | 0.122 |
| Rwork | 0.119 |
| R-free | 0.16500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bkv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.658 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.310 |
| Number of reflections | 78148 | |
| <I/σ(I)> | 15.83 | 4.31 |
| Completeness [%] | 92.9 | 61.6 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
