2BKK
Crystal structure of Aminoglycoside Phosphotransferase APH(3')-IIIa in complex with the inhibitor AR_3a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.670, 98.082, 81.300 |
| Unit cell angles | 90.00, 110.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1MJO 1j7l |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.554 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.150 |
| Rmerge | 0.050 |
| Number of reflections | 46440 |
| Completeness [%] | 97.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15-20 % PEG 5500, 0.1 M MES PH 5.9-6.2 |
