2BJI
High Resolution Structure of myo-Inositol Monophosphatase, The Target of Lithium Therapy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.230, 55.150, 60.880 |
| Unit cell angles | 67.22, 69.65, 85.14 |
Refinement procedure
| Resolution | 10.000 - 1.240 |
| R-factor | 0.153 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hhm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.028 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.100 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.100 | 0.110 |
| Number of reflections | 89508 | |
| <I/σ(I)> | 7.7 | 7.7 |
| Completeness [%] | 84.7 | 41.8 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | ROOM TEMP, VAPOUR DIFFUSION. HANGING DROPS FROM MIXING EQUAL VOLUMES (2 MICRO LITRES) PROTEIN (CONCENTRATION 20 MG/ML BOVINE IMPASE IN 20 MM TRIS.HCL PH 7.5 CONTAINING 20 MM MGCL2) WITH RESERVOIR SOLUTION (0.1 M SODIUM ACETATE, 0.1 M SODIUM HEPES PH 8.5 AND 15% PEG 4000 |
