2BIW
Crystal structure of apocarotenoid cleavage oxygenase from Synechocystis, native enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 119.046, 125.278, 203.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.410 - 2.390 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ACO APO FORM |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.609 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.500 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 111472 | |
| <I/σ(I)> | 10.8 | 2.1 |
| Completeness [%] | 93.3 | 80.9 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | pH 5.70 |
