2BIW
Crystal structure of apocarotenoid cleavage oxygenase from Synechocystis, native enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 119.046, 125.278, 203.086 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.410 - 2.390 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ACO APO FORM |
RMSD bond length | 0.015 |
RMSD bond angle | 1.609 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.500 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.060 | 0.460 |
Number of reflections | 111472 | |
<I/σ(I)> | 10.8 | 2.1 |
Completeness [%] | 93.3 | 80.9 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.7 | pH 5.70 |