2BIV
Crystal structure of the wild-type MBT domains of Human SCML2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.020, 88.206, 186.988 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.1977 |
Rwork | 0.198 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oi1 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.335 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.210 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.180 |
Number of reflections | 69311 | |
<I/σ(I)> | 16.8 | 5.2 |
Completeness [%] | 93.9 | 87.8 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.14M SODIUM IODIDE, 20.5% PEG3350, 15MG/ML PROTEIN |