2BIN
human p53 core domain mutant M133L-H168R-V203A-N239Y-N268D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 45.045, 45.045, 331.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.000 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uol |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 16936 | |
| <I/σ(I)> | 25.5 | 8.6 |
| Completeness [%] | 98.6 | 94.4 |
| Redundancy | 10.2 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
