2BHL
X-RAY STRUCTURE OF HUMAN GLUCOSE-6-PHOSPHATE DEHYDROGENASE (DELETION VARIANT) COMPLEXED WITH GLUCOSE-6-PHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-27 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 117.552, 179.527, 137.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.000 - 2.900 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1QKI CHAIN A B |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | TNT (BUSTER/TNT) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.000 | 3.070 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.120 | 0.410 |
Number of reflections | 32261 | |
<I/σ(I)> | 5.3 | 2 |
Completeness [%] | 98.9 | 92.9 |
Redundancy | 6.8 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 0.1M TRIS, PH 8.5; 0.2M MGCL2; 12% PEG 4000; 5% GLYCEROL |