2BHG
3C protease from type A10(61) foot-and-mouth disease virus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-04 |
Detector | MARRESEARCH |
Spacegroup name | H 3 |
Unit cell lengths | 141.589, 141.589, 43.677 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.760 - 1.900 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.24500 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.800 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.370 |
Number of reflections | 25640 | |
<I/σ(I)> | 12.5 | 2.2 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | SEE PAPER, pH 6.50 |