2BH9
X-RAY STRUCTURE OF A DELETION VARIANT OF HUMAN GLUCOSE 6-PHOSPHATE DEHYDROGENASE COMPLEXED WITH STRUCTURAL AND COENZYME NADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-21 |
Detector | MARRESEARCH |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 60.760, 172.480, 217.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.700 - 2.500 |
R-factor | 0.201 |
Rwork | 0.196 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PARTIALLY-REFINED MONOMER OF HUMAN G6PD CANTON VARIANT |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.700 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.120 | 0.310 |
Number of reflections | 14581 | |
<I/σ(I)> | 9.6 | 3.4 |
Completeness [%] | 73.3 | 59.3 |
Redundancy | 2.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING DROP VAPOUR DIFFUSION. 1-PLUS-1 MICROLITRE DROPS IN THE WELL 0.1M TRIS-HCL,PH 7.5-8.2, 10-16% PEG 4000. PROTEIN CONCENTRATION 5MG/ML. |