2BH2

Crystal Structure of E. coli 5-methyluridine methyltransferase RumA in complex with ribosomal RNA substrate and S-adenosylhomocysteine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-19
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	190.061, 63.542, 112.019
Unit cell angles	90.00, 125.15, 90.00

Refinement procedure

Resolution	50.000 - 2.150
R-factor	0.177
Rwork	0.174
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uwv
RMSD bond length	0.021
RMSD bond angle	2.011
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.090	0.730
Number of reflections	59411
<I/σ(I)>	12.7	1.9
Completeness [%]	99.9	100
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		RUMA-RNA-SAH COMPLEX WAS CRYSTALLIZED FROM 100 MM SODIUM CACODYLATE, PH 6.5, 1.5 M AMMONIUM SULFATE, AND 10 MM MGCL2