2BGQ
apo aldose reductase from barley
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 2003-09-10 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.518, 69.180, 50.468 |
Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
Resolution | 20.310 - 2.500 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ads |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.600 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.400 |
Number of reflections | 15083 | |
<I/σ(I)> | 3.4 | 1 |
Completeness [%] | 96.0 | 96 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 2.4 M (NH4)2SO4, 0.1 M MES PH 6.0 |