2BGQ
apo aldose reductase from barley
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-10 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.518, 69.180, 50.468 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 20.310 - 2.500 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ads |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 15083 | |
| <I/σ(I)> | 3.4 | 1 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2.4 M (NH4)2SO4, 0.1 M MES PH 6.0 |
