2BGC
PrfA-G145S, a constitutive active mutant of the Transcriptional Regulator In L.monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-21 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.247, 100.243, 189.563 |
| Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
| Resolution | 182.570 - 2.300 |
| R-factor | 0.199 |
| Rwork | 0.195 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omi |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.739 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.030 | |
| Number of reflections | 89772 | |
| <I/σ(I)> | 13 | 2.5 |
| Completeness [%] | 92.3 | 94.1 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 12 % (W/V) PEG 8000 100 MM NA-MES PH 6.9 300 MM NA-ACETATE |
