2BFY
Complex of Aurora-B with INCENP and Hesperadin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 287 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.945, 67.044, 116.462 |
| Unit cell angles | 90.00, 96.51, 90.00 |
Refinement procedure
| Resolution | 19.340 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ol5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.010 | 0.020 |
| Number of reflections | 67250 | |
| <I/σ(I)> | 25 | 2.9 |
| Completeness [%] | 96.9 | 79 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS TRIS PROPANE PH 6.6, 18% PEG3350 |
