2BFO
Leishmania major pteridine reductase 1 in complex with NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.308, 103.493, 137.977 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.500 - 2.600 |
R-factor | 0.229 |
Rwork | 0.226 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e92 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.337 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.090 | 0.220 |
Number of reflections | 40607 | |
<I/σ(I)> | 13.6 | 5.5 |
Completeness [%] | 96.8 | 92.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 293 | 15 MG/ML PROTEIN, 0.1M NAAC/HAC BUFFER PH 5.5, 12% MPEG 5000, 0.075M CAAC2, 293K |