2BEX
Crystal structure of Placental Ribonuclease Inhibitor in complex with Human Eosinophil Derived Neurotoxin at 2A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 91.980, 91.980, 257.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.990 |
| R-factor | 0.2059 |
| Rwork | 0.206 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a4y PDB ENTRY 1GQV |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 89545 | |
| <I/σ(I)> | 13.1 | 3.6 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 27.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1M SODIUM MALONATE, PH 7.0 |
