2BEH
Crystal structure of antithrombin variant S137A/V317C/T401C with plasma latent antithrombin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.073, 97.274, 87.388 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 39.100 - 2.700 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 30217 | |
| Completeness [%] | 99.7 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG 4000, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
