2BEE
Complex Between Paromomycin derivative JS4 and the 16S-Rrna A Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2003-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9999 |
Spacegroup name | P 41 |
Unit cell lengths | 39.171, 39.171, 99.027 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
Rwork | 0.220 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7t |
RMSD bond length | 0.006 |
RMSD bond angle | 0.966 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.540 | 2.600 |
Number of reflections | 34100 | |
Completeness [%] | 98.0 | 100 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 310 | MPD, KCl, MGSO4, GLYCEROL, Na Cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | Na Cacodylate | ||
11 | 1 | 2 | H2O | ||
2 | 1 | 1 | KCl | ||
3 | 1 | 1 | MGSO4 | ||
4 | 1 | 1 | GLYCEROL | ||
5 | 1 | 1 | Na Cacodylate | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | MPD | ||
8 | 1 | 2 | KCl | ||
9 | 1 | 2 | MGSO4 |