2BE7

Crystal structure of the unliganded (T-state) aspartate transcarbamoylase of the psychrophilic bacterium Moritella profunda

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-10
Detector	MARRESEARCH
Wavelength(s)	0.92
Spacegroup name	P 32 2 1
Unit cell lengths	129.238, 129.238, 207.232
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.850
R-factor	0.21392
Rwork	0.212
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 6AT1
RMSD bond length	0.011
RMSD bond angle	1.331
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.930
High resolution limit [Å]	2.850	2.850
Number of reflections	45685
Completeness [%]	96.1	97.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	10 mg/ml, 0.1 M Tris, 1.5 M ammonium sulfate , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K