2BE6

2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-11
Detector	ADSC QUANTUM 210
Wavelength(s)	1.116
Spacegroup name	P 1 21 1
Unit cell lengths	84.731, 37.241, 86.860
Unit cell angles	90.00, 97.77, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.205
Rwork	0.202
R-free	0.25500
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.350
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.600	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.052	0.464
Number of reflections	38548
Completeness [%]	97.5	98.9
Redundancy	3.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	284	0.05M Bis-Tris, pH 6.5, 10-15% PEG4000, 0.25mM protein, VAPOR DIFFUSION, HANGING DROP, temperature 284K
1	VAPOR DIFFUSION, HANGING DROP	6.5	284	0.05M Bis-Tris, pH 6.5, 10-15% PEG4000, 0.25mM protein, VAPOR DIFFUSION, HANGING DROP, temperature 284K
1	VAPOR DIFFUSION, HANGING DROP	6.5	284	0.05M Bis-Tris, pH 6.5, 10-15% PEG4000, 0.25mM protein, VAPOR DIFFUSION, HANGING DROP, temperature 284K