2BE1
Structure of the compact lumenal domain of yeast Ire1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.116 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 103.300, 103.300, 401.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.280 - 2.983 |
R-factor | 0.24398 |
Rwork | 0.240 |
R-free | 0.27940 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | Ire1 lumenal domain |
RMSD bond length | 0.026 |
RMSD bond angle | 2.277 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 3.190 |
High resolution limit [Å] | 2.983 | 2.983 |
Rmerge | 0.106 | 0.245 |
Number of reflections | 24150 | |
<I/σ(I)> | 11.8 | 1.8 |
Completeness [%] | 90.8 | 76.3 |
Redundancy | 3.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6 M ammonium sulfate, 5% dioxane, 0.6% MeOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |