2BE1
Structure of the compact lumenal domain of yeast Ire1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 103.300, 103.300, 401.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.280 - 2.983 |
| R-factor | 0.24398 |
| Rwork | 0.240 |
| R-free | 0.27940 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Ire1 lumenal domain |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.277 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 3.190 |
| High resolution limit [Å] | 2.983 | 2.983 |
| Rmerge | 0.106 | 0.245 |
| Number of reflections | 24150 | |
| <I/σ(I)> | 11.8 | 1.8 |
| Completeness [%] | 90.8 | 76.3 |
| Redundancy | 3.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6 M ammonium sulfate, 5% dioxane, 0.6% MeOH, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
