2BDW
Crystal Structure of the Auto-Inhibited Kinase Domain of Calcium/Calmodulin Activated Kinase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2004-12-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.121, 77.104, 119.520 |
| Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
| Resolution | 45.800 - 1.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2phk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 73357 | |
| Completeness [%] | 99.4 | 83.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 1.6 M sodium malonate, 1.5 % 1,2,3-heptanetriol, pH 7.0, VAPOR DIFFUSION, temperature 293K |
