2BDG
Human Kallikrein 4 complex with nickel and p-aminobenzamidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0500 |
| Spacegroup name | P 4 |
| Unit cell lengths | 95.680, 95.680, 38.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.950 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | hk6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.010 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 25621 | |
| <I/σ(I)> | 15.06 | 3.25 |
| Completeness [%] | 98.6 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG-MME 2000, 100 mM Tris, 10 mM nickel chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
