2BCV
DNA polymerase lambda in complex with Dttp and a DNA duplex containing an unpaired Dtmp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-08 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.140, 62.988, 141.936 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.190 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | STRUCTURE (STUDIO) |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 34418 | |
| Completeness [%] | 99.2 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Sodium citrate, sodium cacodylate, 2-propanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium citrate | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | 2-propanol | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Sodium citrate | ||
| 6 | 1 | 2 | sodium cacodylate |
