2BCU
DNA polymerase lambda in complex with a DNA duplex containing an unpaired Damp and a T:T mismatch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-10-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.998, 62.667, 140.494 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.030 - 2.200 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rzt |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | STRUCTURE (STUDIO) |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.180 | 2.180 |
Number of reflections | 25468 | |
Completeness [%] | 98.7 | 87.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Sodium citrate, sodium cacodylate, 2-propanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Sodium citrate | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | 2-propanol | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | Sodium citrate | ||
6 | 1 | 2 | sodium cacodylate |