2BCU

DNA polymerase lambda in complex with a DNA duplex containing an unpaired Damp and a T:T mismatch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-10-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	55.998, 62.667, 140.494
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.030 - 2.200
R-factor	0.219
Rwork	0.219
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rzt
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	STRUCTURE (STUDIO)
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.180	2.180
Number of reflections	25468
Completeness [%]	98.7	87.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	Sodium citrate, sodium cacodylate, 2-propanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	Sodium citrate
2	1	1	sodium cacodylate
3	1	1	2-propanol
4	1	1	H2O
5	1	2	Sodium citrate
6	1	2	sodium cacodylate