2BCQ
DNA polymerase lambda in complex with a DNA duplex containing an unpaired Dtmp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.96318 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.044, 62.539, 139.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.740 - 1.650 |
| R-factor | 0.206 |
| Rwork | 0.201 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | STRUCTURE (STUDIO) |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 57247 | |
| Completeness [%] | 94.8 | 78.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Sodium citrate, sodium cacodylate, 2-propanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium citrate | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | 2-propanol | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Sodium citrate | ||
| 6 | 1 | 2 | sodium cacodylate |
