2BCN
Solvent isotope effects on interfacial protein electron transfer between cytochrome c and cytochrome c peroxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-03-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.533, 47.089, 84.289 |
| Unit cell angles | 102.47, 98.74, 102.16 |
Refinement procedure
| Resolution | 19.610 - 1.700 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.967 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 70156 | |
| Completeness [%] | 93.3 | 67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 295 | PEG3350, NaCl, BOG, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
