2BCK
Crystal Structure of HLA-A*2402 Complexed with a telomerase peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.1 |
Synchrotron site | SRS |
Beamline | PX14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.488 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.505, 132.186, 91.197 |
Unit cell angles | 90.00, 128.80, 90.00 |
Refinement procedure
Resolution | 78.570 - 2.800 |
R-factor | 0.187 |
Rwork | 0.183 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ao7 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.348 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.620 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.153 | 0.917 |
Number of reflections | 28450 | |
<I/σ(I)> | 3.3 | 1.2 |
Completeness [%] | 99.4 | 99.7 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 1.6M Ammonium Sulphate, 0.1M Sodium Chloride, 0.1M HEPES, 10% v/v Dioxane, pH 7.0, VAPOR DIFFUSION, temperature 293K |