2BBW
Crystal structure of human adenylate kinase 4 (AK4) in complex with diguanosine pentaphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.836, 66.375, 77.380 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 32.359 - 2.050 |
| R-factor | 0.18323 |
| Rwork | 0.180 |
| R-free | 0.24892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zd8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.833 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 25968 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 94.7 | 69.4 |
| Redundancy | 4.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG 6000, 0.2M NaCl, 10% Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
