2BB2

X-RAY ANALYSIS OF BETA B2-CRYSTALLIN AND EVOLUTION OF OLIGOMERIC LENS PROTEINS

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	77.800, 83.600, 109.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.100
R-factor	0.186
Rwork	0.186
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.100 *
Rmerge	0.063 *
Number of reflections	18000 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	Tris-acetate	0.05 (M)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	sodium azide	0.02 (%(w/v))
5	1	reservoir	methanepentanediol	35 (%(v/v))
6	1	reservoir	calcium acetate	18 (mM)