2BAS
Crystal Structure of the Bacillus subtilis YkuI Protein, with an EAL Domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.335, 125.271, 167.962 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.040 - 2.610 |
| R-factor | 0.20678 |
| Rwork | 0.203 |
| R-free | 0.28700 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.257 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.044 | 0.340 |
| Number of reflections | 30096 | |
| <I/σ(I)> | 17.54 | 3.7 |
| Completeness [%] | 94.7 | 84.7 |
| Redundancy | 2.97 | 2.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.2M Sodium Acetate thrihydrate, 0.1M Tris Hydrochloride, 30% PEG 4000., pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
