2BA0

Archaeal exosome core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	101.570, 129.590, 102.330
Unit cell angles	90.00, 101.56, 90.00

Refinement procedure

Resolution	20.000 - 2.700
Rwork	0.216
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.380
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	69958
Completeness [%]	98.1	84.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293.15	0.1M HEPES, 35% MPD, 10mM CaCl2, 15% Glycerol, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
1	VAPOR DIFFUSION, SITTING DROP	6.2	293.15	0.1M HEPES, 35% MPD, 10mM CaCl2, 15% Glycerol, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K