2B9R
Crystal Structure of Human Cyclin B1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 117.169, 117.169, 197.685 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.24928 |
| Rwork | 0.243 |
| R-free | 0.30795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fin |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.363 |
| Data reduction software | DENZO |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.380 |
| Number of reflections | 18101 | |
| <I/σ(I)> | 12.7 | 4.8 |
| Completeness [%] | 98.1 | 99.7 |
| Redundancy | 6.39 | 6.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
