2B9H

Crystal structure of Fus3 with a docking motif from Ste7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.116
Spacegroup name	P 21 21 21
Unit cell lengths	58.300, 63.788, 99.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.550
Rwork	0.187
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b9f
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.550
Number of reflections	52829
<I/σ(I)>	26.6
Completeness [%]	96.9
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	298	15-20% PEG8000, 0.1M MES pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K