2B8P
Crystal structure of Acanthamoeba polyphaga mimivirus NDK, the first viral nucleoside diphosphate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97563 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 99.425, 99.425, 99.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.120 - 2.550 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k44 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.360 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.059 | 0.256 |
| Number of reflections | 8922 | |
| <I/σ(I)> | 9.7 | 2.9 |
| Completeness [%] | 95.9 | 94.1 |
| Redundancy | 4.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Ammonium sulfate 1.6M, MES 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
