2B69
Crystal Structure of Human UDP-glucoronic acid decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 46.000, 45.000, 85.000 |
| Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.210 |
| R-factor | 0.13559 |
| Rwork | 0.134 |
| R-free | 0.16203 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.506 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.370 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 93023 | |
| Completeness [%] | 91.3 | 58.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | PEG3350, Zn (ac)2, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
