2B5Z
Hen lysozyme chemically glycosylated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.580, 32.970, 120.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.880 - 1.600 |
| Rwork | 0.212 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iee |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.570 | 1.600 |
| High resolution limit [Å] | 1.510 | 1.500 |
| Rmerge | 0.039 | 0.250 |
| Number of reflections | 19330 | |
| <I/σ(I)> | 22.19 | 6.78 |
| Completeness [%] | 95.3 | 79.6 |
| Redundancy | 5.17 | 5.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | Magnesium Sulfate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
