2B5L
Crystal Structure of DDB1 In Complex with Simian Virus 5 V Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.758, 240.792, 117.177 |
| Unit cell angles | 90.00, 101.79, 90.00 |
Refinement procedure
| Resolution | 47.800 - 2.850 |
| R-factor | 0.2304 |
| Rwork | 0.229 |
| R-free | 0.29880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b5m |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.800 |
| High resolution limit [Å] | 2.630 |
| Number of reflections | 92110 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20000, sodium chloride, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
