2B56

Structural Basis for UTP Specificity of RNA Editing TUTases From Trypanosoma Brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Collection date	2005-01-10
Spacegroup name	P 41 21 2
Unit cell lengths	91.435, 91.435, 162.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.970
R-factor	0.19398
Rwork	0.193
R-free	0.22086
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.011
RMSD bond angle	1.506
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.050
High resolution limit [Å]	1.970	1.970
Rmerge	0.066	0.641
Number of reflections	49388
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.0