2B51
Structural Basis for UTP Specificity of RNA Editing TUTases from Trypanosoma Brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Collection date | 2004-12-22 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.006, 91.006, 162.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.050 |
| R-factor | 0.18895 |
| Rwork | 0.187 |
| R-free | 0.22734 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.514 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.020 |
| Number of reflections | 43582 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 3 M KCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
