2B4H
Crystal Structure of the Rhesus Rotavirus VP5 Antigen Domain Dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.99980 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 163.356, 54.805, 65.734 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.500 - 1.600 |
R-factor | 0.2039 |
Rwork | 0.203 |
R-free | 0.22450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Residues 267-470 of VP5CT (1SLQ) |
RMSD bond length | 0.012 |
RMSD bond angle | 1.611 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 73654 | |
<I/σ(I)> | 28.36 | 4.54 |
Completeness [%] | 93.6 | 69.9 |
Redundancy | 4.7 | 4.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298.15 | Tris, sodium chloride, EDTA, MPD, Glycerol, sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |