2B4H
Crystal Structure of the Rhesus Rotavirus VP5 Antigen Domain Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99980 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 163.356, 54.805, 65.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 1.600 |
| R-factor | 0.2039 |
| Rwork | 0.203 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Residues 267-470 of VP5CT (1SLQ) |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.611 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 73654 | |
| <I/σ(I)> | 28.36 | 4.54 |
| Completeness [%] | 93.6 | 69.9 |
| Redundancy | 4.7 | 4.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298.15 | Tris, sodium chloride, EDTA, MPD, Glycerol, sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
