2B48

Bcl-XL 3D Domain Swapped Dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-12-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	92.645, 92.645, 96.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.910 - 3.450
R-factor	0.261
Rwork	0.261
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r2d
RMSD bond length	0.008
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.550
High resolution limit [Å]	3.450	3.450
Rmerge	0.080	0.676
Number of reflections	5544
<I/σ(I)>	24.39	2.18
Completeness [%]	93.5	79.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	277	100mM Na/K Phosphate, 42% 2-Methyl-2,4 Pentanediol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K