2B3Z
Crystal structure of a bifunctional deaminase and reductase involved in riboflavin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.322, 108.450, 186.977 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.410 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27700 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.440 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.056 | 0.349 |
| Number of reflections | 67740 | |
| <I/σ(I)> | 17.7 | 3.9 |
| Completeness [%] | 97.6 | 93.2 |
| Redundancy | 9.9 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 26.6% polyethylene glycol 400, 5% glycerol, 190mM MgCl2, 95mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
