2B3H
Crystal structure of Human Methionine Aminopeptidase Type I with a third cobalt in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.290, 77.300, 48.340 |
| Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.100 |
| R-factor | 0.0983 |
| Rwork | 0.101 |
| R-free | 0.13100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yj3 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.038 | 0.197 |
| Number of reflections | 142947 | |
| <I/σ(I)> | 60.37 | 4.7 |
| Completeness [%] | 91.8 | 78.9 |
| Redundancy | 3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 2000, potassium chloride, hepes, sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
