2B3D
Crystal structure of Modulator of Drug activity B in complex with flavin adenine dinucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.626, 84.105, 55.490 |
| Unit cell angles | 90.00, 110.95, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| R-factor | 0.1933 |
| Rwork | 0.193 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2amj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.950 | 0.291 |
| Number of reflections | 23420 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 97.7 | 84.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, Calcium Chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
