2B2U
Tandem chromodomains of human CHD1 complexed with Histone H3 Tail containing trimethyllysine 4 and dimethylarginine 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-08 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.984, 54.753, 100.274 |
| Unit cell angles | 90.00, 112.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.950 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b2t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.860 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.500 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.111 | |
| Number of reflections | 11356 | |
| <I/σ(I)> | 12 | 2.11 |
| Completeness [%] | 94.5 | 70.5 |
| Redundancy | 3.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 283 | 4% PEG3350, 0.05M HEPES, pH 8.0, 10mM BTP, 12.5mM NaCl, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 283K, pH 8.00 |
